Then he moved as post-doctoral fellow to the University of California, San Francisco, USA, where he became interested in vascular development and in health and diseased angiogenesis. We foresee the zebrafish model as an important system that can possibly complement well-established mouse models in cancer research to generate novel insights into the molecular mechanism of the tumor angiogenesis. To identify the cause, we performed genetic, in vitro and in vivo studies. NA differentiation is normal in cloche mutants that are devoid of endothelial cells but have VMCs. We further show that TGFÎ², zeb1a and foxo1a are tightly linked together by miR-145 In iSMC-committed cells, TGFÎ² induces the expression of miR-145, which in turn is able to downregulate zeb1a and foxo1a The absence of miR-145 results in only a slight reduction in the number of iSMCs, which still express mesenchymal genes but fail to contract. In summary, we have identified a hemodynamic-dependent mechanism in the developing vasculature that controls vMC recruitment. Angiogenesis, from the Greek blood vessels (angio) creation (genesis), is an important biological process that occurs both during health and disease. Grave lutto per Rita Dalla Chiesa dopo il dolore per Fabrizio Frizzi: qualche giorno fa, pare a causa di una Genome-wide SNP typing and parametric linkage analysis was done in a recessive model to identify candidate regions. Moreover, we show that the CARD is required to suppress cell proliferation and migration. These aspects are particularly relevant in cardiovascular tissues wherein alterations of the redox balance are associated with stroke, aging, and pharmacological intervention. Our team has recently identified novel metabolic pathways that are crucial for tumor survival and cancer progression (more information available upon request). The Alk5-dependent induction of zeb1a and foxo1a is required for this morphogenetic process: zeb1a is responsible for driving LPM migration around the gut, whereas foxo1a regulates LPM predisposition to iSMC differentiation. FASHIONING BLOOD VESSELS BY ROS SIGNALLING AND METABOLISM. Â©2018 BY SANTORO LAB. Get in touch with us for any questions or comments regarding our work and publications. Santoro Massimo* PO Pat. The purpose of this review is to provide an overview of redox mechanisms, sources and antioxidants that control signaling events in ECs. Tumor cells harbor genetic alterations that promote a continuous and elevated production of reactive oxygen species. In this framework, PPFIA1 interacts with active Î±5Î²1 integrin and localizes close to EC adhesions where post-Golgi carriers are targeted. Here, we use the zebrafish embryo to ask whether the DA is required for SN development. We identify a pathway, comprising the regulators of post-Golgi carrier formation PI4KB and AP-1A, the small GTPase Rab11B, the surface tyrosine phosphatase receptor PTPRF and its adaptor PPFIA1, which we propose acts as a funnel combining FN secretion and recycling of active Î±5Î²1 integrin from the trans-Golgi network (TGN) to the EC surface, thus allowing FN fibrillogenesis. Recent advances arising from in vitro strategies for deriving mural cells from human pluripotent stem cells as well as from the zebrafish model will be discussed and the medical relevance of these discoveries will be highlighted. His lab is currently focused on studying how redox signaling, metabolism, and hemodynamic forces regulates endothelial homeostasis in development as well as during cancer progression. Here we show that, on Rab21-elicited internalization, FN-bound/active Î±5Î²1 is recycled to the EC surface. Massimo Santoro, morto all’età di 52 anni, era originario di Foggia ma viveva a Roma da molti anni. Last, we will discuss the potential therapeutic use of agents that directly or indirectly block metabolism. Aveva 70 anni. Despite their inherent proximity to circulating oxygen and nutrients, endothelial cells (ECs) oxidize only a minor fraction of glucose in mitochondria, a metabolic specialization that is poorly understood. An ambiguous aspect of redox biology is how redox events occur in subcellular organelles including mitochondria, and nuclei. Generale 3037 Mar. 14.30-17 (Ed.19) email@example.com Veneziani Bianca Maria PO Pat. Massimo’s goal is to expand the current vision of endothelial biology by identifying unforeseen metabolic and signaling mechanisms and used them to develop novel anti-angiogenesis therapies to treat cancer progression and diabetic conditions. Tra questi anche suo genero, Massimo Santoro, scomparso a soli 52 anni stroncato da una grave malattia. The function of hemodynamic forces in controlling vMC recruitment is unclear. Highly recommended as a CTO for startup-type enterprises. Exome sequencing analysis was done in unaffected and affected siblings. Here we describe a protocol for fast and reliable (13)C-isotope-based in vivo metabolic profiling. A key question in vascular biology is how the diversity of origin of vascular mural cells, namely smooth muscle cells and pericytes influences vessel properties, in particular the regional propensity to vascular diseases. We will also highlight the potential of zebrafish for identifying antitumor angiogenesis drugs to block tumor development and progression. Using transgenic lines marking developing vMCs in zebrafish embryos, we find that vMCs are recruited by arterial-fated vessels and that the process is flow dependent. SANTORO LAB Department of Biology, University of Padua, Italy View Massimo Santoro’s profile on LinkedIn, the world's largest professional community. Massimo has 6 jobs listed on their profile. Con il suo gruppo Massimo Santoro studia i meccanismi molecolari e cellulari alla base della formazione e del mantenimento del sistema cardiovascolare e dei processi di stress ossidativo. During his career, he has been granted with the EMBO (2004) and HFSP (2005) long-term fellowships, the HFSP Career Developmental Award (2008), the Marie Curie Reintegration Grant (2010), the Odysseus-FWO Career Grant (2014), and, more recently, the ERC Consolidator Grant (2016). Santoro MM, Beltrame M, Panakova D, Siekmann A, Tiso N, Venero Galanternik M, Jung HM, Weinstein B Nano-Sampling and Reporter Tools to Study Metabolic Regulation in Zebrafish FRONT CELL DEV BIOL In this review we discuss how ROS-mediated signalling events and distinctive metabolic pathways drive the biology of endothelial cells. Such studies offer unique opportunities for designing new therapeutic strategies against metastasis and cancer spreading. ROLE OF AMINO ACID METABOLISM IN ANGIOGENESIS. CILIA CONTROL VASCULAR MURAL CELL RECRUITMENT IN VERTEBRATES. These findings identify UBIAD1 as a nonmitochondrial CoQ10-forming enzyme with specific cardiovascular protective function via the modulation of eNOS activity. Join Facebook to connect with Massimo Santoro and others you may know. Intestinal smooth muscle cells (iSMCs) are a crucial component of the adult gastrointestinal tract and support intestinal differentiation, peristalsis and epithelial homeostasis during development. Si è infatti spento all’età di soli 52 anni Massimo Santoro, noto non solo per essere il genero di Rita Dalla Chiesa, ma anche per aver a lungo lavorato come autore di “Uno Mattina”, uno dei programmi di punta di casa Rai. Nuovo lutto per la famiglia di Rita Dalla Chiesa. Sia il 2017 che il 2018, infatti, sono stati caratterizzati da dolorose assenze. The candidate must be a highly motivated researcher in a relevant discipline as well as having some experience in genetic, molecular, and/or cellular biology methods. In this review, we will discuss why the zebrafish model system has the advantage of being a vertebrate model equipped with easy and powerful transgenesis as well as imaging tools to investigate not only physiological angiogenesis but also tumor angiogenesis. We then consider the evidence that connects heterogeneities in smooth muscle cell and pericyte origins with disease. Vascular mural cells (vMCs) are essential components of the vertebrate vascular system, controlling blood vessel maturation and homeostasis. DATA ON METABOLIC-DEPENDENT ANTIOXIDANT RESPONSE IN THE CARDIOVASCULAR TISSUES OF LIVING ZEBRAFISH UNDER STRESS CONDITIONS. A stroncarlo un male incurabile. Nelle ultime ore è morto Massimo Santoro, il genero di Rita Dalla Chiesa. In the linkage regions, we selected candidate genes that harbor two rare variants with predicted functional effects in the patients and for which the unaffected sibling is either heterozygous or homozygous reference. Excellent communication skills in spoken and written English are required. The recent availability of novel technologies and organisms to model cell metabolism in vivo is expanding current knowledge of cell metabolism. Generale 3758 Mar. Rita Dalla Chiesa ricorda il genero Massimo Santoro, morto alcuni mesi fa. Since this idea has major implications for understanding and modelling human disease, then there is a pressing need for new model systems to investigate mural cell development and the consequences of heterogeneity. Nel corso della sua carriera aveva lavorato a Quelli della notte con Renzo Arbore, passando poi ad altri programmi e facendosi apprezzare, anno dopo anno, per le sue doti da autore televisivo. In this context, the zebrafish (Danio rerio) is emerging as a valuable model system to learn about the metabolic routes critical for cellular homeostasis. 14.30-17 (Ed.19) firstname.lastname@example.org SSD MED/04 CFU 5 Anno di corso III Semestre II Insegnamenti propedeutici previsti: Nessuno RISULTATI DI APPRENDIMENTO ATTESI Conoscenza e capacità di comprensione Recent and new tools to investigate physiological ROS signaling will be also discussed. PPFIA1 DRIVES ACTIVE Î5Î1 INTEGRIN RECYCLING AND CONTROLS FIBRONECTIN FIBRILLOGENESIS AND VASCULAR MORPHOGENESIS. See the complete profile on LinkedIn and discover Massimo’s connections and jobs at similar companies. This review therefore first describes the role and regulation of mural cells during vascular formation, with a focus on embryonic origin. ©2018 BY SANTORO LAB. It is envisioned that this small vertebrate system will help in the understanding of pathogenesis for numerous metabolic-related disorders in humans and in the identification of their therapeutic treatments. In particolare, si occupa di una rara malattia oculare in cui questi processi … The analysis and the interpretation of the data can be found in the associated article . Loss of UBIAD1 reduces the cytosolic pool of the antioxidant CoQ10 and leads to ROS-mediated lipid peroxidation in vascular cells. Thus, PDGFR-mediated mural cell recruitment mediates neurovascular interactions between the aorta and sympathetic precursors and promotes their noradrenergic differentiation. 13C-ISOTOPE-BASED PROTOCOL FOR PRENYL LIPID METABOLIC ANALYSIS IN ZEBRAFISH EMBRYOS. Rita Dalla Chiesa in lacrime e dolorante: il lutto sempre vivo We find that loss of PKM2 alters mitochondrial substrate utilization and impairs EC proliferation and migration in vivo. BLOOD FLOW CONTROLS BONE VASCULAR FUNCTION AND OSTEOGENESIS. Due to this, Aurora B remains trapped on chromatin in dividing cells and fails to translocate to the spindle midzone during cytokinesis. Con il suo gruppo Massimo Santoro studia i meccanismi molecolari e cellulari alla base della formazione e del mantenimento del sistema cardiovascolare e dei processi di stress ossidativo. In 2017, he returned to Italy at University of Padua as Chair of Cell Biology at Department of Biology. We combine genetic and metabolic approaches coupled to advance imaging techniques to address specific biological questions and develop new therapeutic approaches. Human UBIAD1 is a nonmitochondrial prenyltransferase that synthesizes CoQ10 in the Golgi membrane compartment. Rita Dalla Chiesa sta per tornare in tv dopo un periodo tremendo: la morte del genero e la malattia dell’ex marito Fabrizio Frizzi. Often, the same mechanisms are responsible for the insurgence of vascular-associated pathologies. In this article we used transgenic zebrafish lines that express compartment-specific isoforms of the roGFP2-Orp1 and Grx1-roGFP2 biosensors, described in Panieri et al (2017) , to test the contribute of the pentose phosphate pathway and of the glutathione biosynthesis in the antioxidant capacity of myocardial and endothelial cells in vivo. LOSS OF PYRUVATE KINASE M2 LIMITS GROWTH AND TRIGGERS INNATE IMMUNE SIGNALING IN ENDOTHELIAL CELLS, NEW MODELS TO STUDY VASCULAR MURAL CELL EMBRYONIC ORIGIN: IMPLICATIONS IN VASCULAR DISEASES. Massimo Mattia Santoro obtained is Master Diploma from University of Turin, Italy and his PhD in Molecular and Cellular Biology from the Open University, London, UK. Here, we demonstrate that iSMCs arise from the lateral plate mesoderm (LPM) in a stepwise process. Furthermore, S-adenosylmethionine synthesis is impaired in the absence of PKM2, resulting in DNA hypomethylation, de-repression of endogenous retroviral elements (ERVs) and activation of antiviral innate immune signalling. PROUDLY CREATED WITH WIX.COM, Department of Biology, University of Padua, Italy, LABORATORY OF ANGIOGENESIS AND REDOX METABOLISM, EXPLORING THE WONDERS OF BLOOD VESSELS AND CELLULAR METABOLISM. Basolateral polymerization of cellular fibronectin (FN) into a meshwork drives endothelial cell (EC) polarity and vascular remodelling. Learn more about our team and areas of study below. In 2008, he established his first independent laboratory at the University of Torino, opening the Molecular Biotechnology Center. ROS HOMEOSTASIS AND METABOLISM: A DANGEROUS LIASON IN CANCER CELLS. Such findings are providing an overview of the ROS biology relevant for endothelial cells in the context of normal and pathological angiogenic conditions. In aged mice, skeletal blood flow and endothelial Notch activity are also reduced leading to decreased angiogenesis and osteogenesis, which is reverted by genetic reactivation of Notch. Combining pharmacological and genetic approaches, we show that TGFÎ²/Alk5 signaling drives the LPM ventral migration and commitment to an iSMC fate. Rogfp2-based transgenic zebrafish lines represent valuable tools to characterize the impact of redox changes in living tissues and offer new opportunities for studying metabolic driven antioxidant response in biomedical research. Cellular inhibitor of apoptosis (cIAP) proteins are E3 ligases that modulate diverse biological processes such as cell survival, proliferation, and migration. Santoro era molto conosciuto nella cittÃ termale dove viveva. Dai geni alla terapia: nuovi farmaci antitumorali. In particolare, si occupa di una rara malattia oculare in cui questi processi … Metabolism has a decisive role in many fundamental biological processes, including organism development and tissue homeostasis. Precise morphogenetic and cellular mechanisms act in endothelial cells (ECs) to drive angiogenesis during growth and throughout adulthood. Discrete molecular mechanisms have been associated with vMC development and differentiation. AN EXCLUSIVE CELLULAR AND MOLECULAR NETWORK GOVERNS INTESTINAL SMOOTH MUSCLE CELL DIFFERENTIATION IN VERTEBRATES. In this review we focus on the metabolism of those amino acids and the intermediates that hold an established function within angiogenesis and endothelial pathophysiology. UBIAD1 IS AN ANTIOXIDANT ENZYME THAT REGULATES ENOS ACTIVITY BY COQ10 SYNTHESIS, CARD-MEDIATED AUTOINHIBITION OF CIAP1'S E3 LIGASE ACTIVITY SUPPRESSES CELL PROLIFERATION AND MIGRATION. Congenital or neonatal cardiomyopathies are commonly associated with a poor prognosis and have multiple etiologies. Mechanistically, we show that the NF-ÎºB transcription factor RELB is responsive to PKM2 loss, limiting EC growth through the regulation of P53. COMPOUND HETEROZYGOUS LOSS-OF-FUNCTION MUTATIONS IN KIF20A ARE ASSOCIATED WITH A NOVEL LETHAL CONGENITAL CARDIOMYOPATHY IN TWO SIBLINGS. Intravital imaging reveals that vessel growth in murine long bone involves the extension and anastomotic fusion of endothelial buds. Targeting of molecular pathways involved in such tumor angiogenetic processes by using specific drugs or inhibitors is important for developing new anticancer therapies. Whereas such oxidative stress conditions would be harmful to normal cells, they facilitate tumor growth in multiple ways by causing DNA damage and genomic instability, and ultimately, by reprogramming cancer cell metabolism. While blood vessels play important roles in bone homeostasis and repair, fundamental aspects of vascular function in the skeletal system remain poorly understood. Here we show that the long bone vasculature generates a peculiar flow pattern, which is important for proper angiogenesis. Here, we have solved the structure of the caspase recruitment domain (CARD) of cIAP1 and identified that it is required for cIAP1 autoregulation. In particular, we describe which molecules are involved in redox signaling and how they influence the relationship between ECs and other vascular component with regard to angiogenesis. This procedure represents a valuable approach for researchers interested in studying the effect of gene manipulation on lipid metabolism in zebrafish and in understanding the genetic conditions that result in metabolism dysfunction. REAL-TIME QUANTIFICATION OF SUBCELLULAR H2O2 AND GLUTATHIONE REDOX POTENTIAL IN LIVING CARDIOVASCULAR TISSUES. We demonstrate that the CARD inhibits activation of cIAP1's E3 activity by preventing RING dimerization, E2 binding, and E2 activation. The process of de novo vessel formation, called angiogenesis, is essential for tumor progression and spreading. We also discuss recent work which provides a rationale for specific amino acid-restricted diets and its beneficial effects on vascular tissues, including extending the life span and preventing the development of a variety of diseases. Inhibition of platelet-derived growth factor receptor (PDGFR) signaling prevents VMC differentiation and also blocks NA differentiation of SN precursors. ROS SIGNALING AND REDOX BIOLOGY IN ENDOTHELIAL CELLS. This review outlines the metabolic-dependent mechanisms that tumors engage in when faced with oxidative stress conditions that are critical for cancer progression by producing redox cofactors. These probes detect hydrogen peroxide (H2O2) levels and glutathione redox potential (EGSH), both with high spatiotemporal resolution. Unraveling how E3 ligases are regulated is important because miscontrolled ubiquitylation can lead to disease. In two siblings, a male and female, we identified an undescribed type of lethal congenital restrictive cardiomyopathy affecting the right ventricle. Here we show that the glycolytic enzyme pyruvate kinase M2 (PKM2) limits glucose oxidation, and maintains the growth and epigenetic state of ECs. Here, the most recent methods and studies on cell metabolism are summarized, which support the overall value for the zebrafish model system not only to study metabolism but also metabolic disease states. Ecco le sue dichiarazioni. Thank you for your interest in our research. We propose that blood flow and endothelial Notch signalling are key factors controlling ageing processes in the skeletal system. News 5 … This is a crucial biological event in all vertebrates. ZEBRAFISH AS A MODEL TO EXPLORE CELL METABOLISM, ANTIANGIOGENIC CANCER DRUG USING THE ZEBRAFISH MODEL. The role of endothelial metabolism represents a crucial element governing the formation and the differentiation of blood vessels, termed angiogenesis. PROUDLY CREATED WITH WIX.COM, Department of Biology, University of Padua, Italy. He worked as a post-doc fellow at University of California, San Francisco, USA, with Prof. Dr. Didier Stainier, where he became interested in cardiovascular development and endothelial homeostasis. Massimo M. Santoro obtained is Master Diploma from University of Turin, Italy and his PhD in Molecular and Cellular Biology from the Open University, London, UK. Further, CARD-mediated autoregulation is also necessary to maximally suppress caspase-8-dependent apoptosis and vascular tree degeneration in vivo. Il saluto commovente di Uno Mattina a Massimo Santoro. Massimo Santoro, autore Tv, se ne è andato a 52 anni, era sposato con Giulia Cirese, figlia di Rita Dalla Chiesa, conosciuta nella redazione di Uno Mattina. Together, our data uncover a cascade of molecular events that govern distinct morphogenetic steps during the emergence and differentiation of vertebrate iSMCs. We offer a dynamic work environment in a stimulating scientific surrounding with a young,enthusiastic, motivated team along the opportunity to contribute to high-impact projects. Massimo aveva 52 anni e faceva parte dello staff di autori di Uno Mattina, noto programma televisivo.Proprio l’ex conduttrice di Forum ha diffuso, sabato scorso, la luttuosa notizia sul suo profilo Facebook: ‘Ciao, Massimo. Primary cilia are microtubule-based organelles that protrude from the membrane of many cells, including neurons, osteoblasts, epithelial, and endothelial cells (ECs).1 In vertebrates, primary cilia function as a signaling platform by detecting extracellular stimuli and initiating intracellular signaling response.2 Besides having chemosensory function, primary cilia are best known as mechanosensors in some cells. The 405/488 nm ratio was normalized to the initial value obtained before oxidants addition and plotted over time. Primary cilia are microtubule-based organelles that protrude from the membrane of many cells, including neurons, osteoblasts, epithelial, and endothelial cells (ECs). We provide evidence for the usefulness of these Tg lines for pharmacological compounds screening by addressing the blocking of pentose phosphate pathways (PPP) and glutathione synthesis, thus altering subcellular redox state in vivo. Ã deceduto in un ospedale di Roma ieri pomeriggio. Massimo SANTORO. However, mechanisms coordinating Î±5Î²1 integrin-mediated extracellular FN endocytosis and exocytosis of newly synthesized FN remain elusive. View the profiles of people named Massimo Santoro. We identified a novel autosomal recessive congenital restrictive cardiomyopathy, caused by a near complete loss-of-function of KIF20A. Drug discovery remains to be the main focus for biomedical research and represents the essence of antiangiogenesis cancer research. The sympathetic nervous system controls smooth muscle tone and heart rate in the cardiovascular system. 1 person has recommended Massimo Join now to view View Massimo’s full profile See who you know in common Get introduced Contact Massimo directly Join to view full profile Massimo Santoro ha illustrato come le scoperte della biologia e della genetica hanno aperto nuove strade per la cura del cancro, delineando il ruolo dei geni nell’insorgenza dei tumori e le nuove possibilità di individuare strategie terapeutiche. Protection against oxidative damage caused by excessive reactive oxygen species (ROS) by an antioxidant network is essential for the health of tissues, especially in the cardiovascular system. This finding further illustrates the relationship of cytokinesis and congenital cardiomyopathy. The past few years have seen the zebrafish system emerge as a valid model organism to study developmental angiogenesis and, more recently, as an alternative vertebrate model for cancer research. E3 ligases mediate the covalent attachment of ubiquitin to target proteins thereby enabling ubiquitin-dependent signaling. Surprisingly, inhibition of eNOS prevents Ubiad1-dependent cardiovascular oxidative damage, suggesting a crucial role for this enzyme and nonmitochondrial CoQ10 in NO signaling. Prima di affrontare il problema dell’abuso e del maltrattamento è opportuna una premessa su cosa vuol dire fare e come è stata fatta la storia dell’infanzia. We identified two compound heterozygous variants in KIF20A; a maternal missense variant (c.544C>T: p.R182W) and a paternal frameshift mutation (c.1905delT: p.S635Tfs*15). The protocol takes 5-7 d to complete, requiring minimal equipment and analytical expertise, and it represents a unique alternative to the existing ex vivo (e.g., cell lines) isotope-based metabolic methods. This protocol can be applied to different types of experimental settings, including tissue-specific metabolic analyses or dynamic metabolic changes that occur during vertebrate embryogenesis. Detecting and measuring the dynamic redox events that occur in vivo is a prerequisite for understanding the impact of oxidants and redox events in normal and pathological conditions. We hypothesized a novel autosomal recessive condition. Morto Massimo Santoro, genero di Rita Dalla Chiesa e autore di Uno Mattina A soli 52 anni è morto l’autore Rai, marito della figlia della Dalla Chiesa, Giulia Cirese. Cell metabolism plays a key role in many essential biological processes. Despite these crucial roles, the origin of iSMCs and the mechanisms responsible for their differentiation and function remain largely unknown in vertebrates. Here, we identified a gene with important antioxidant features by analyzing a null allele of zebrafish ubiad1, called barolo (bar). This work reveals the metabolic and functional consequences of glucose oxidation in the endothelium, highlights the importance of PKM2 for endothelial growth and links metabolic dysfunction with autoimmune activation in ECs. Taken together, our data reveal mechanisms by which the E3 ligase activity of cIAP1 is controlled, and how its deregulation impacts on cell proliferation, migration and cell survival. This protocol covers the loading of isotope precursor; extraction, preparation and quantification of the labeled lipid metabolites (e.g., the prenyl lipid CoQ10) by the means of HPLC-MS; and its analysis in zebrafish embryos. Besides glycolysis and fatty acid oxidation, endothelial cells rely on specific amino acids to proliferate, migrate, and survive. The laboratory of Prof. Massimo Santoro offers a PhD position to study redox homeostasis and metabolism in cancer. A novembre dello scorso anno è venuto a mancare Massimo Santoro, marito della figlia di Rita, Giulia Cirese. 'Massimo ha lottato fino all'ultimo e Giulia con lui. bar mutants show specific cardiovascular failure due to oxidative stress and ROS-mediated cellular damage. Functional studies confirmed that the R182W mutation creates an ATPase defective form of KIF20A which is not able to support efficient transport of Aurora B as part of the chromosomal passenger complex. We show that noradrenergic (NA) differentiation of SN precursors temporally coincides with vascular mural cell (VMC) recruitment to the DA and vascular maturation. Abuso e maltrattamento nell’infanzia: implicazioni psicologiche per un conselling efficace. Santoro, M.M., Beltrame, M., Panáková, D., Siekmann, A.F., Tiso, N., Venero Galanternik, M., Jung, H.M., Weinstein, B.M. VASCULAR MURAL CELLS PROMOTE NORADRENERGIC DIFFERENTIATION OF EMBRYONIC SYMPATHETIC NEURONS. Incontro con Massimo Santoro. Massimo Mattia Santoro obtained is Master Diploma from University of Turin, Italy and his PhD in Molecular and Cellular Biology from the Open University, London, UK. After sequential excitation at 405 nm and 488 nm the emission was collected between 500-520 nm every 2 min for an overall duration of 60 min. Our laboratory focuses on the identification of novel molecular mechanisms and signaling pathways involved in formation of blood vessels in developmental and pathological conditions. Impaired blood flow leads to defective angiogenesis and osteogenesis, and downregulation of Notch signalling in endothelial cells. Postganglionic sympathetic neurons (SNs) develop in close proximity to the dorsal aorta (DA) and innervate visceral smooth muscle targets. Non sono ore facili per Rita Dalla Chiesa e per la sua famiglia, colpita da un grave lutto che coinvolge anche la Rai. Massimo is an inventive and organizational mind. Formation and maturation of a functional vascular network is a process called angiogenesis. We take advantage of tissue-specific CRISPR gene targeting to demonstrate that hemodynamic-dependent Notch activation and the ensuing arterial genetic program is driven by endothelial primary cilia. In particular, we describe how the mitochondria has a key role in regulating the interplay between redox homeostasis and metabolism within tumor cells. Ã morto lâ ex presidente delle Terme di Fiuggi, il professor Massimo Santoro. The transgenic zebrafish embryos were subdued to metabolic inhibition and subsequently challenged with H2O2 or the redox-cycling agent menadione to respectively mimic acute or chronic oxidative stress. The laboratory, located in the Department of Biology at the University of Padua, strives to understand blood vessels biology and cancer progression. We also identify zebrafish foxc1b as a cilia-dependent Notch-specific target that is required within endothelial cells to drive vMC recruitment. Confocal time-lapse recordings were performed to follow the compartmentalized H2O2 and EGSH changes in the cardiovascular tissues of zebrafish embryos at 48 h post fertilization. Blood flow and angiogenesis in aged mice are also enhanced on administration of bisphosphonate, a class of drugs frequently used for the treatment of osteoporosis. The laboratory of Massimo Santoro at University of Padua strives to understand blood vessels biology and cell metabolism in pathological angiogenesis and cancer progression. Genetically-encoded Rogfp2 fluorescent probes have become powerful tools for real-time detection of redox events.